Cloned (Comment) | Organism |
---|---|
into the plasmid pT7 for expression in Escherichia coli B834DE3 pLacIRARE2 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of apo-YfaU is determined to 1.39 A resolution and the structure of a Mg2+-pyruvate product complex to 1.93 A resolution | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
4-hydroxy-2-ketohexanoic acid | data for Ni2+-activated YfaU | Escherichia coli | |
0.078 | - |
2-dehydro-3-deoxy-L-rhamnonic acid | data for Mg2+-activated YfaU | Escherichia coli | |
0.1 | - |
4-hydroxy-2-ketoheptane-1,7-dioate | data for Ni2+-activated YfaU | Escherichia coli | |
0.1 | - |
4-hydroxy-2-ketopentanoic acid | data for Ni2+-activated YfaU | Escherichia coli | |
0.14 | - |
2-keto-3-deoxy-L-mannonate | data for Mg2+-activated YfaU | Escherichia coli | |
0.15 | - |
4-hydroxy-2-ketoheptane-1,7-dioate | data for Mg2+-activated YfaU | Escherichia coli | |
0.8 | - |
2-keto-3-deoxy-L-lyxonate | data for Mg2+-activated YfaU | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Ni2+ | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P23522 | - |
- |
Purification (Comment) | Organism |
---|---|
using metal affinity and size exclusion chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-L-rhamnonate | - |
Escherichia coli | ? | - |
? | |
2-keto-3-deoxy-L-lyxonate | - |
Escherichia coli | ? | - |
? | |
2-keto-3-deoxy-L-mannonate | - |
Escherichia coli | ? | - |
? | |
4-hydroxy-2-ketoheptane-1,7-dioate | - |
Escherichia coli | pyruvate + succinic semialdehyde | - |
? | |
4-hydroxy-2-ketohexanoic acid | - |
Escherichia coli | ? | - |
? | |
4-hydroxy-2-ketopentanoic acid | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxy-2-ketoheptane-1,7-dioate aldolase | - |
Escherichia coli |
HpcH | - |
Escherichia coli |
YfaU | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-keto-3-deoxy-L-lyxonate | data for Mg2+-activated YfaU | Escherichia coli | |
0.3 | - |
2-keto-3-deoxy-L-mannonate | data for Mg2+-activated YfaU | Escherichia coli | |
0.4 | - |
2-dehydro-3-deoxy-L-rhamnonic acid | data for Mg2+-activated YfaU | Escherichia coli | |
0.54 | - |
4-hydroxy-2-ketoheptane-1,7-dioate | data for Mg2+-activated YfaU | Escherichia coli | |
299 | - |
4-hydroxy-2-ketoheptane-1,7-dioate | data for Ni2+-activated YfaU | Escherichia coli | |
396 | - |
4-hydroxy-2-ketopentanoic acid | data for Ni2+-activated YfaU | Escherichia coli | |
447 | - |
4-hydroxy-2-ketohexanoic acid | data for Ni2+-activated YfaU | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Escherichia coli |